Ken Caldwell, director of Save the Children's international operations, said: "We deplore this brutal killing of humanitarian workers in Darfur. Our deepest sympathies are with the family and friends of our Sudanese colleagues."
International monitors from the African Union, which is trying to maintain a cease-fire between the government and the rebels, recovered the bodies and are investigating the deaths.
An aid worker was shot on the same road in the summer but survived. In October, two Save the Children workers were killed in north Darfur by a landmine.
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